目的 探讨辅酶Q10对环磷酰胺大鼠皮质骨的影响,并与阳性药物阿伦磷酸钠进行比较。 方法 32只3月龄SPF级♂SD大鼠,随机分为正常对照组(CON)、环磷酰胺组(CTX)、阿仑膦酸钠组(ALD)、辅酶Q10组(CoQ10),连续给药15 d。实验结束后,骨组织形态计量学观测左侧胫骨中段骨磨片的动静态参数。同时,取右侧股骨进行骨生物力学检测。 结果 与正常对照组相比,环磷酰胺可明显抑制骨形成,使得大鼠皮质骨变薄,骨髓腔扩大,新骨矿化形成减少;股骨脆性增加,骨折的风险显著增加。与环磷酰胺组相比,辅酶Q10 不仅可有效地促进骨形成,抑制环磷酰胺诱导的大鼠骨丢失,且骨生物力学参数最大强度、断裂应变、韧性系数、刚性系数均得到明显的修复。结论 辅酶Q10 (30 mg·kg-1·d-1)对环磷酰胺大鼠胫骨中段骨和股骨皮质骨的微观结构有较好的修复能力。
Abstract
OBJECTIVE To investigate the preventive effects of coenzyme Q10 on cortical bone in cyclophosphamide-treated rats and compare CoQ10 with alendronate sodium. METHODS Thirty-two three-month-old SPF and SD male rats were randomly divided into 4 groups (n=8 per group). Group 1 was treated with vehicle as the control group (CON group).Other groups were treated with cyclophosphamide (4.5 mg·kg-1·d-1) first, and then vehicle (CTX group), alendronate sodium (ALD 1 mg·kg-1·d-1) and CoQ10(30 mg·kg-1·d-1) once a day for 15 d. At the experimental endpoint, The static and dynamic parameters of left tibia bone grinding by the bone histomorphometry and took the right femur bone biomechanical testing were observated. RESULTS From bone histomorphometry parameters, cyclophosphamide can inhibit bone formation which make rat cortical bone thinning, bone marrow cavity to expand; reduced bone formation. Coenzyme Q10 can effectively promote bone formation, inhibit bone loss in rats by cyclophosphamide, the effect is better than the positive drug alendronate sodium. Compared with the CON group, biomechanical properties of maximal strength, break strength, break strain and toughness index were decreased significantly, while modulus of rigidity was increased significantly in the CTX group. Compared with the CTX group, the ALD group, only several parameters of the biomechanical properties were significantly improved. In contrast, in the CoQ10 group, the biomechanical properties were significantly improved. CONCLUSION CoQ10 (30 mg·kg-1·d-1) on the microstructure of CTX rat tibia bone repair capacity better, and the ability to repair bone mass and reduce the risk of hip fracture is also superior ALD.
关键词
骨质疏松 /
辅酶Q10 /
环磷酰胺 /
骨生物力学 /
骨组织形态计量学
{{custom_keyword}} /
Key words
osteoporosis /
coenzyme Q10 /
cyclophosphamide /
biomechanical peoperty /
bone histomorphometry
{{custom_keyword}} /
中图分类号：
R965
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] MICHAUD L B, COODIN S. Cancer-treatment-induced bone loss, partl . Am J Health Syst Pharm, 2003,63(5):419-430.
[2] WU T, LIU Y Y, CUI L, et al. Effects of different doses of cyclophosphamide on bone pharmacology in male rats. Chin Pharmacol Bull(中国药理学通报), 2001,17(3):329-333.
[3] YU Q, WU T, CUI L, et al. Effect of Danshen Gubao on bone histomorphometry in cyclophosphamide-induced bone loss of rats. Chin J Osteoporosis(中国骨质疏松杂志), 2007,13(5):308-312,351.
[4] ZHANG Q Y, QIN L P, HUANG B K, et al. Study on effects of total coumarines from the fruits of Cnidium monnieri on osteoporosis in ovariectomized rats. Chin Pharm J(中国药学杂志), 2003,38(2): 101-103.
[5] MOON H J, KO W K. Antioxidants, like coenzyme Q10, selenite, and curcumin, inhibited osteoclast differentiation by suppressing reactive oxygen species generation. Biochem Biophys Res Commun,2012,418(2):247-253.
[6] HOJIN M, WANKYU K, MINSEO J, et al. Coenzyme Q10 regulates osteoclast and osteoblast differentiation. J Food Sci, 2013,78(4):785-791.
[7] QIAN X, WANG Z Q, HAN G P, et al. The pharmacology and clinical application of coenzyme Q10. Food Drug A(食品与药品A), 2006,8(1):16-19.
[8] WU Q Y, XIONG X T, XU B L, et al. Effects of prednisone on trabecular microstructure and biomechanical properties of femur in a rat model of type II collagen-induced arthritis . Chin Pharmacol Bull(中国药理学通报), 2014,30(7):1018-1022.
[9] ST-TU Y L, KE X, L S M, et al. The observation and evaluation of bone histomorphometry in three osteoporosis models of mice. Chin Pharmacol Bull(中国药理学通报), 2014,30(9):1281-1287.
[10] CHEN M, ZHENG Q, FANG Z H, et al. Effects of alendeonate on boen resorption and osteoclast like cells formation in mouse bone marrow cluture. Orthopedic J China(中国矫形外科杂志), 2008,16(4):282-284.
[11] ZHANG X Z, SONG L G, LI H, et al. Effects of alendronate on bone mineral density, cytokines and indices of bone metabolism in postmenopausal osteoporotic patients. Chin J Osteoporosis(中华内科杂志),2006,45(7):565-568.
[12] ZHANG L J, QU J F, BAI Y J, et al. Protective effect of coenzyme Q10 on retinal pigment epithelial cell from oxidative stress injury . Chin J Exp Ophthalmol(中华实验眼科杂志), 2013,32(11):1037-1044.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
湛江市科技计划项目(2012C3104017); 广东省高等教育教学改革项目(GDJG20142230)
{{custom_fund}}
PDF(931 KB)
